| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.027564 |
| Chromosome: | chromosome 7 |
| Location: | 4595594 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344200 | (1 of 3) IPR000104//IPR020846 - Antifreeze protein, type I // Major facilitator superfamily domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCAGCGGCCAGCTCCAGCGCGTAGACCCGCCGAAGGCTGCTGCTGCT |
| Internal bar code: | ATAATTCTGTAGCTTCCGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 258 |
| LEAP-Seq percent confirming: | 31.25 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCGGGTTCTGTCCATCTC |
| Suggested primer 2: | CACGCACACACACACTCTTG |