Insertion junction: CLIP2.027567_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.027567
Chromosome:chromosome 9
Location:5025743
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g801065 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCATCAAACGGGAGCGCGACATAATCACATCCGGGACGCCGATGCCAT
Internal bar code:CTTGCTGAGGACGTGTGTAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1486
LEAP-Seq percent confirming:94.1176
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:ACCAACAAACTGCTCACCCA
Suggested primer 2:CCACCGCCACTTTTCATGTG