Insertion junction: CLIP2.027571_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.027571
Chromosome:chromosome 10
Location:68960
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g417900 (1 of 89) 3.6.4.13 - RNA helicase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTACCGCTACCCTCGCCAGTGCCAGTGCCGCCACCACCAACTTCGGCGG
Internal bar code:GACCAGACCACAGTACATGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2587
LEAP-Seq percent confirming:79.3651
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTGCACCACACATGTTC
Suggested primer 2:ACGTCGCTAGCAAACAGTCA