| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.027576 |
| Chromosome: | chromosome 6 |
| Location: | 4242030 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278249 | AST5 | Putative aspartate aminotransferase; (1 of 1) PTHR11751//PTHR11751:SF33 - SUBGROUP I AMINOTRANSFERASE RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTGGTGGACTTGTCCATTGGCGCCAGCGACCTCGCGCCCCCGCCCGA |
| Internal bar code: | CATCTTTTGTTACGTATAGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 137 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGAATCAAGCAGCAGCAC |
| Suggested primer 2: | ACTATCTGCAAAGTGCCCCC |