| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.027589 |
| Chromosome: | chromosome 12 |
| Location: | 4920667 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524150 | POLH2,POLI2 | (1 of 2) K03509 - DNA polymerase eta (POLH); DNA polymerase eta | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGGCTAATGTTTTTGGGCTCATGGACCGTGGAGTGGCGTGGGCAAGG |
| Internal bar code: | GATGACTTCGGGCCATGCTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1732 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCAAAGGCGACAAGAGA |
| Suggested primer 2: | CGTCCTCTCGCACTCCATC |