| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.027608 |
| Chromosome: | chromosome 6 |
| Location: | 1680256 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g262350 | (1 of 1) IPR003722 - Cobalamin (vitamin B12) biosynthesis CobH/CbiC, precorrin-8X methylmutase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCACCGCTACGGCTGTTGCCGCTGGCTCCGCCGGAACCGCCGCAGCAC |
| Internal bar code: | GTTGTGCTGAATTTTACCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 740 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCACGCGCAAAACAAGAG |
| Suggested primer 2: | GTCTTGCAGACCACTCGACA |