| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.027613 |
| Chromosome: | chromosome 14 |
| Location: | 297070 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609600 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGCGCCGTGTGTGTCCATGTGTGTGTGTGTGTGTGTGTGTGTGTGTG |
| Internal bar code: | TTCAGTGTTTTGTTGCCATCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1997 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCATCGACGATCATGGA |
| Suggested primer 2: | GTCTGCTGCATGTTTAGCGG |