| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.027617 |
| Chromosome: | chromosome 1 |
| Location: | 5057129 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035200 | (1 of 13) 2.3.1.225 - Protein S-acyltransferase / S-protein acyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTGCATTGCCGCTACTGTGACCGGTGCGTGCTGGGCTTTGACCACCA |
| Internal bar code: | CGGATAGTAGGCGAGTCCTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1250 |
| LEAP-Seq percent confirming: | 81.4815 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCGGTGATGACTAGGAAG |
| Suggested primer 2: | ACACCTTGCTCTCCAGAAGC |