Insertion junction: CLIP2.027624_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.027624
Chromosome:chromosome 9
Location:2666710
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g392000 (1 of 3) PF09763 - Exocyst complex component Sec3 (Sec3_C) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCAGACCCAGGTGGTCGCTGGGCGCCAGACTGCGCACGTCGTCGTGCT
Internal bar code:TAAGGCAACTCCTTTTGTGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:430
LEAP-Seq percent confirming:82.7586
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCCCTCCCGTAATCATGC
Suggested primer 2:TGTTGAAGGGGTCGTACGTG