| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.027628 |
| Chromosome: | chromosome 14 |
| Location: | 505110 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611250 | POC9 | Proteome of centriole protein 9; (1 of 2) PTHR12086:SF9 - EF-HAND DOMAIN-CONTAINING PROTEIN 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCTGGCACGGGGGCGCCTGCCCAAGACGCTGCCGCCCGTATGTGAG |
| Internal bar code: | CCTGTTCAGCTAGTCTTGACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 787 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCGTAGTACTGCAGCTGC |
| Suggested primer 2: | ACGTTGCTCCTGGAAGTCAG |