Insertion junction: CLIP2.027637_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.027637
Chromosome:chromosome 7
Location:764917
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g317850 FBB10 Flagellar and Basal Body 10; (1 of 1) IPR029582 - Basal body-orientation factor 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCGTACTGCGGCGCGCCCTGGTGTGTGTGCTTGCCGTGCAGCTCGCAG
Internal bar code:CCTACGTTTGGCCGGGTCACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:3206
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCACACAATCCTGCATAG
Suggested primer 2:GAACTGCACAAAAGCGAGCA