Insertion junction: CLIP2.027655_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.027655
Chromosome:chromosome 14
Location:2517285
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g624450 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTCCACCACCAACCTCGGCGCCTACGCCAATGCCCACGCGAACGCCGC
Internal bar code:GCCGGGGGTGTTACGGGAATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:275
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GGATGAAGTGCTGACGTCCA
Suggested primer 2:ACACAACACCGGTTCCTTCA