| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.027673 |
| Chromosome: | chromosome 6 |
| Location: | 7996578 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304650 | PIK3 | Phosphatidylinositol 3-kinase; (1 of 1) PTHR10048:SF22 - PHOSPHATIDYLINOSITOL 4-KINASE BETA | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGGCATAACTAGGCTGCTTCTTGCGCAGCAAGCAGTGGCGGGCAGGAC |
| Internal bar code: | CGCGCATGTAATTGTGTAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3820 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGCTGCTGTTGTTGTT |
| Suggested primer 2: | TTTTGTGGATGCGCTGTGTG |