| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.027679 |
| Chromosome: | chromosome 8 |
| Location: | 3618415 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379750 | TMEM216,MKS2 | Transmembrane protein 216; (1 of 1) K19385 - transmembrane protein 216 (TMEM216) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGGAAAGGGCCCTTATGAGGCGGTTTCGGATTGCAATAGGTACGCCCC |
| Internal bar code: | TGAATCCTGAACGCTTCTACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3535 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAGATCCCGCGCCTATTC |
| Suggested primer 2: | GGAGATGGTCCAAAGGGGTG |