| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.027709 |
| Chromosome: | chromosome 3 |
| Location: | 7547536 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205250 | ELG4 | Exostosin-like glycosyltransferase 4; (1 of 39) PTHR11062//PTHR11062:SF21 - EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAATGTAACCCCCCCCCCACACACACACACACACACACACACACATGC |
| Internal bar code: | CGCACTGTAAAGGTCATTCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1190 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGATGGGAGCATGAACAGT |
| Suggested primer 2: | AGTGCGACTGTTTCCCTACG |