Insertion junction: CLIP2.027713_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.027713
Chromosome:chromosome 2
Location:7702942
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g147100 FAP265 Flagellar Associated Protein 265; (1 of 7) PF05517 - p25-alpha (p25-alpha) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCATGCCTGCGTGCTCATGTGTTTGCCTCGCCTCGCTCTCGCTGCAGG
Internal bar code:GTTCGATATTGCGTTTCTACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1059
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCTCGATACGCCCTGGAC
Suggested primer 2:GCTTTGCTACCATCATGCCG