| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.027765 |
| Chromosome: | chromosome 14 |
| Location: | 2606249 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625150 | PGM8 | (1 of 1) PTHR23029:SF26 - PROTEIN Y18H1A.4-RELATED; Phosphoglycerate mutase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTACTGCCGCTACTGCCGTTGTTTCTACTGCCACTCCCGAAATGCCGGC |
| Internal bar code: | TTTCAACACCGCCACAGCTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1842 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGTGTTGCGAATTGTGC |
| Suggested primer 2: | GCATCCACTACGGCAGCTAT |