Insertion junction: CLIP2.027770_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.027770
Chromosome:chromosome 9
Location:113202
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g386550 (1 of 1) K12492 - ADP-ribosylation factor GTPase-activating protein 1 (ARFGAP1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGAGAAGCTGCGTGCGGATGTGGAGGGCCGCGACTACACGCCGCCCTC
Internal bar code:GTCCGATAAACCATGATGTGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:3162
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCAGGTTGGTCAGATCGT
Suggested primer 2:CACTCGATCGGCTACCAGTC