| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.027781 |
| Chromosome: | chromosome 7 |
| Location: | 2711136 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331100 | PHC11 | Putative pherophorin-chlamydomonas homolog | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAGATATTTCGTTTGCCTCTTCGTGTCACTAGCTCATGGCGCGCGCGC |
| Internal bar code: | TCGATAGACCAGCTGAAAAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4578 |
| LEAP-Seq percent confirming: | 97.6562 |
| LEAP-Seq n confirming: | 125 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 128 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCACACACTCGCAGATCT |
| Suggested primer 2: | AGCCTATCGGTTGCGATCTG |