Insertion junction: CLIP2.027787_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.027787
Chromosome:chromosome 8
Location:2144595
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g369800 DHS2 Deoxyhypusine synthase; (1 of 2) 2.5.1.46 - Deoxyhypusine synthase / Spermidine dehydrogenase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCCATCTGTTCCAATACGTTGGGATGGTTTAGTTTATGCATGGGAATG
Internal bar code:TTACACTGCCCATCTATGCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:3264
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:153
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:153

Suggested primers for confirmation by PCR

Suggested primer 1:CAACCCGTGCTGTGAACTTG
Suggested primer 2:GGCTTCTAGCACTGCCTGAA