Insertion junction: CLIP2.027792_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.027792
Chromosome:chromosome 16
Location:971673
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g648750 (1 of 1) PF10373 - Est1 DNA/RNA binding domain (EST1_DNA_bind) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGCGTGTGTGTGGGCGTGTGCGTGTGTATGTGGGCGTGTTTTTGGGGT
Internal bar code:TTGGGATGTAATAATGCGGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1615
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGCTGCAGTGGGTGTTTG
Suggested primer 2:TGGAAGCAAGGTGAGAGCAG