Insertion junction: CLIP2.027806_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.027806
Chromosome:chromosome 2
Location:6076054
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g119300 (1 of 1) PF14368 - Probable lipid transfer (LTP_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCCATTGTGTGTTCCCTTCCTAAACACGCTTGCGCAACCCCGCCCCCC
Internal bar code:CCTCAGGGCATACTGCGTTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:2728
LEAP-Seq percent confirming:98.75
LEAP-Seq n confirming:79
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:TAACCTCCTCTCCTCCTGCC
Suggested primer 2:CTTCCGCAATTGCACACACA