Insertion junction: CLIP2.027812_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.027812
Chromosome:chromosome 16
Location:5375750
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g683250 (1 of 1) PF05832 - Eukaryotic protein of unknown function (DUF846) (DUF846) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAGTGAAGCTGGTGGGCTGCAAGCCAGTTAGGGAGCCACGCTGCGCAT
Internal bar code:CGCCGTATATTACCACCCAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2150
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGGATCATTTGCGCACTG
Suggested primer 2:CGATCGCATGCAAAGAAGGG