| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.027819 |
| Chromosome: | chromosome 12 |
| Location: | 1180076 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490550 | CDS | ||
| Cre12.g490600 | (1 of 2) IPR009060//IPR015940 - UBA-like // Ubiquitin-associated domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCAGTCGAAGTTGGCCGAAATCCGCGCCAAAGCTAGTAATACAGCAT |
| Internal bar code: | CATGTGGATCTGGAACAGGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 945 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACTGACCCTCACCTGACA |
| Suggested primer 2: | CTGTACAGGCGGGTGACTTT |