Insertion junction: CLIP2.027827_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.027827
Chromosome:chromosome 12
Location:9903271
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g541352 LIP1 (1 of 1) PTHR11005//PTHR11005:SF17 - LYSOSOMAL ACID LIPASE-RELATED // AB-HYDROLASE ASSOCIATED LIPASE REGION CONTAINING PROTEIN; Putative triacylglycerol lipase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGAGCCCAGCAAACAATCGCCCACAGCCACTCCCACATCGCGCCAGTGG
Internal bar code:GAGGGGGTTAGCTCTATATTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3875
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:57
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAGGACACCTCCACACTC
Suggested primer 2:CCACCTTAGCGTCCTCGATC