| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.027830 |
| Chromosome: | chromosome 13 |
| Location: | 1942211 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576200 | (1 of 3) PF01048 - Phosphorylase superfamily (PNP_UDP_1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATTTCTCCATGTAACTTTATTGCACGCGCCAAATGCGTTGGAAGTTTG |
| Internal bar code: | CGTACTTCTTACCTGTTCTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2278 |
| LEAP-Seq percent confirming: | 84.375 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCACACCCATACCCATCC |
| Suggested primer 2: | GTCCACACCATTTGCTGCAG |