| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.027836 |
| Chromosome: | chromosome 1 |
| Location: | 6395487 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g046650 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGCAAGCAAGGGCGGGCGAGGTAGCTTTGCAAGAGCAGATGGTAAAC |
| Internal bar code: | CATTCTATACAATCCAAGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5068 |
| LEAP-Seq percent confirming: | 95.9596 |
| LEAP-Seq n confirming: | 95 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 99 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAGACTCCTTAGCCTCCA |
| Suggested primer 2: | AAGCACTAGTCACTGGTGGC |