Insertion junction: CLIP2.027845_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.027845
Chromosome:chromosome 2
Location:882710
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g078950 MMP17 Matrix metalloproteinase; (1 of 40) 3.4.24.38 - Gametolysin / Lysin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGACGACCCTACACGCCACGCCACGTCTCGGCACCTAAACCAATGCAC
Internal bar code:TTAGGATTTTGTGGGACTACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1133
LEAP-Seq percent confirming:60.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTAGGGTACGTGCCACTA
Suggested primer 2:GGCGCACAACAAAGTCGATT