| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.027862 |
| Chromosome: | chromosome 2 |
| Location: | 5660115 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115850 | (1 of 3) PF01987 - Mitochondrial biogenesis AIM24 (AIM24) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACCTGATGAGTGCTTGGGAGGGCCCAAACCACATCATTGCGGCCTGC |
| Internal bar code: | CTGTACACTAGGATAATCGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3741 |
| LEAP-Seq percent confirming: | 73.3333 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTAGCAATCGCGATGCCA |
| Suggested primer 2: | CCAGATGCTACCAGCTACGG |