Insertion junction: CLIP2.027880_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.027880
Chromosome:chromosome 3
Location:7360189
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g206500 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACACGGACAGGCGCCGGCCGCACCCACCCGCCAGCACGGAGTCGTACTC
Internal bar code:TTGTTAAAGTCGGCGAGAAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:5746
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:85
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:85

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTGTCTGTCAGCTTCCTG
Suggested primer 2:CACACACACACATGCACAGG