Insertion junction: CLIP2.027918_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.027918
Chromosome:chromosome 7
Location:3920575
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g339300 (1 of 3) PF05180 - DNL zinc finger (zf-DNL) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCTGCTCGGAAGAGGGGGTATTCGACATCGCCAGGGTCCGCACCGCGC
Internal bar code:GAGCCTACTATTTCCTAAAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2818
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGCACACACAGTCGCATA
Suggested primer 2:ACACCTGTTGGATGAGGCTG