| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.027970 |
| Chromosome: | chromosome 12 |
| Location: | 5898846 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533550 | PYK1 | (1 of 1) PTHR11817:SF5 - PYRUVATE KINASE-RELATED; Pyruvate kinase 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCGGCAGGTTCACGTTCTTGCGCTCGCTGCACGGGCACAGGGCGAGGG |
| Internal bar code: | GGCCCTCAGTTTGGCCGGGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1411 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTACTCAGGGGTGGGTG |
| Suggested primer 2: | TTCTCGGTGGGGATCTCCAT |