Insertion junction: CLIP2.027975_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.027975
Chromosome:chromosome 6
Location:3702033
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278154 (1 of 1) PTHR31560:SF0 - UPF0652 PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGACATCTTCGAGCTGGGGCGACGATACAAGATCCTCAACCCGGACAA
Internal bar code:TGGTTCCAGGGTGCCAATACGT

Confirmation - LEAP-Seq

LEAP-Seq distance:6130
LEAP-Seq percent confirming:98.3607
LEAP-Seq n confirming:60
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCAGAGCATCGATACGGG
Suggested primer 2:CTGGGTCCGTAGCTAGCATG