| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.027979 |
| Chromosome: | chromosome 12 |
| Location: | 3057163 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501403 | MLO2 | (1 of 1) PF03094//PF13833 - Mlo family (Mlo) // EF-hand domain pair (EF-hand_8); MLO family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGGATCTGCGGCCGCCGGAACCAGAACAGCGCCCGCAGCTTGCGCTG |
| Internal bar code: | TGGTAGGATTTCTCCGTTTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1654 |
| LEAP-Seq percent confirming: | 88.5714 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCCAAGGTGTTCAGCCTG |
| Suggested primer 2: | ACGACGCCGGAGTTACTTAC |