| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.027991 |
| Chromosome: | chromosome 7 |
| Location: | 5065543 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g347800 | SMM29 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) PTHR14614:SF30 - S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGATAAGGCGAGGGCGTGTCTTGGGACCATGGGCTGGAGTAGCGGAC |
| Internal bar code: | TGTCTAGTAAAATACTGCGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2545 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAACTGTTTGCGACGCAG |
| Suggested primer 2: | ACTTTCCTAGTGCACGGGTG |