| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.028009 |
| Chromosome: | chromosome 14 |
| Location: | 1478176 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617400 | HSP22F | Heat shock protein 22F; (1 of 4) PTHR11527//PTHR11527:SF128 - SMALL HEAT-SHOCK PROTEIN HSP20 FAMILY // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAAGGTCGGACCGGCAGCATCGACCGCATGGCGATGGCGCGAGCGCCGG |
| Internal bar code: | GTTCTCTCGGTGAGACCTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1756 |
| LEAP-Seq percent confirming: | 96.8254 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGCTTGAGCAAGGTGTG |
| Suggested primer 2: | GAAGGAAAACCCGTTCGCAC |