| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.028048 |
| Chromosome: | chromosome 16 |
| Location: | 7051029 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674515 | IDA7,DIC3,IA1-IC140,IC140 | (1 of 1) PTHR12442//PTHR12442:SF5 - DYNEIN INTERMEDIATE CHAIN // WD REPEAT-CONTAINING PROTEIN 63; Flagellar Inner Arm Dynein I1/f Intermediate Chain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGAAGGACAGCGACATGATGGGCGTGGCGGCCAGCGTGACGCGGATGG |
| Internal bar code: | GCCTACCTATAGATTTGCTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5631 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGCCGATTTCGTCAAGC |
| Suggested primer 2: | CGGTGAGTGAAGGTCAGCTT |