| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.028053 |
| Chromosome: | chromosome 6 |
| Location: | 3929984 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278196 | (1 of 3) PF01575 - MaoC like domain (MaoC_dehydratas) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCACATGCGCGCCACTCTCCCGCCCCATACACGCACATGCACACCAAG |
| Internal bar code: | AAGACGTACTGTGGATTCCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3131 |
| LEAP-Seq percent confirming: | 86.2069 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAAACACAGTCACTGCG |
| Suggested primer 2: | TGGCTCTACCTACAGGCCAT |