| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.028065 |
| Chromosome: | chromosome 1 |
| Location: | 5513390 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039200 | FAP9,Rabl5,IFT22 | (1 of 1) K07935 - Rab-like protein 5 (RABL5); Intraflagellar transport protein 22 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGAAATGTCATACCAGCCTACGGCTGCAGTCAGGTCAGTGGTGTCGC |
| Internal bar code: | ACTAAAGTCTAAAAACCCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2436 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGTTGGGCTCTGCAAAG |
| Suggested primer 2: | AGGGAGTGGTACAGGCTCAT |