| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.028095 |
| Chromosome: | chromosome 3 |
| Location: | 5838142 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g188100 | (1 of 8) IPR001810//IPR006553 - F-box domain // Leucine-rich repeat, cysteine-containing subtype | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCACGACTCACCGCCGCAGGTGTCCAACGCGGGTCTCCGCGGCCTGG |
| Internal bar code: | GACACGATTGAGCGATATAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 178 |
| LEAP-Seq percent confirming: | 6.25 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACGGGTTGTGCATCAGG |
| Suggested primer 2: | CAATGGTGCAGGAGCTGGTA |