Insertion junction: CLIP2.028139_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.028139
Chromosome:chromosome 10
Location:3085885
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g441300 (1 of 11) IPR001471//IPR016177//IPR031112 - AP2/ERF domain // DNA-binding domain // AP2-like ethylene-responsive transcription factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGATCTGGGTCTGGGTGACATGAGCGACATCGACGAGGCAGCCCTAGC
Internal bar code:ATGATCGCCAGGACCTGTGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:831
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCCAGCCGACTCAGATACTC
Suggested primer 2:TGCTCACAATGCAGGGAGAG