| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.028140 |
| Chromosome: | chromosome 13 |
| Location: | 4044630 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g590650 | (1 of 1) PF07223 - Protein of unknown function (DUF1421) (DUF1421) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGTCACTTTCTCGGCATTCTCCGAGCGAGTCGCTGCACCAGAACCGCT |
| Internal bar code: | CAGTAGCAGGACTGTGCCATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 681 |
| LEAP-Seq percent confirming: | 36.0656 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACGGCTGCAAAGTTGTGC |
| Suggested primer 2: | CTGCTGGTACTGTGGGTAGC |