Insertion junction: CLIP2.028140_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.028140
Chromosome:chromosome 13
Location:4044634
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g590650 (1 of 1) PF07223 - Protein of unknown function (DUF1421) (DUF1421) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTCTCGGCAGACGACCTCGCTGCGCTCGTGGATAAGGGCGTGCATGCT
Internal bar code:CAGTAGCAGGACTGTGCCATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2003
LEAP-Seq percent confirming:73.913
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTGGTACTGTGGGTAGC
Suggested primer 2:TTACGGCTGCAAAGTTGTGC