Insertion junction: CLIP2.028143_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.028143
Chromosome:chromosome 3
Location:6272550
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g191850 SDR8 Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.184//1.1.1.300 - Carbonyl reductase (NADPH) / Xenobiotic ketone reductase // NADP-retinol dehydrogenase / Retinol dehydrogenase (NADP(+)) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAATTGATGTGCATGCAAACGGGGGCCTCTTCGCACCTGGGCTGTGTTGA
Internal bar code:TAGCCTTGCTCAGTACATTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1992
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:ACCACACACACCTTGACGTT
Suggested primer 2:CGTATCAAAGCGGAAAGGCG