Insertion junction: CLIP2.028147_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.028147
Chromosome:chromosome 12
Location:9703695
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g555803 NDA6 Type-II NADH dehydrogenase; (1 of 5) 1.6.5.9 - NADH:ubiquinone reductase (non-electrogenic) / Ubiquinone reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGCGTTTCATTATTGCCATCATCAACCGGCCTGCCGCTGCGAACGCAA
Internal bar code:GGAGGGCGGGTATGGTCCCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:656
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTATCTACAACCCCCACGCC
Suggested primer 2:ACGCATCACACAAACACACG