Insertion junction: CLIP2.028184_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.028184
Chromosome:chromosome 7
Location:2406650
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g328800 NSG13 (1 of 8) PF00514 - Armadillo/beta-catenin-like repeat (Arm); Protein expressed during nitrogen-starved gametogenesis intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAAGTGTCTAACCCCGGTGCCGCACCACCTCCGTCAATACACTACGTGC
Internal bar code:TGGTTTTGCGTCTAAAATTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:300
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCAACCAATCGCGCCTTTAA
Suggested primer 2:ATCAACGTCGTCCACCACTC