Insertion junction: CLIP2.028194_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.028194
Chromosome:chromosome 14
Location:2144354
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g621800 GT90-27,GT90F27 GT90 family protein 27; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTCAGTTTTGGGTTTATCGGACTTCGGACTTGAGGTGACGAAGGGTGG
Internal bar code:GTCGATGACTCGCAATTTTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3696
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:90
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:90

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAAACTCGCAGATCCCAT
Suggested primer 2:GCTTTATGCCTTGAGACGCG