Insertion junction: CLIP2.028225_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.028225
Chromosome:chromosome 16
Location:3075732
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g664400 (1 of 2) PF00235 - Profilin (Profilin) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCTCTGCAGGCACTCTTTAATGACCGCGACGACGCCATTAAGCATGGC
Internal bar code:TATGGTGAGCGGTAGTTCCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2442
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:69
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:69

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCGTAGGTTCCATACGGC
Suggested primer 2:CCCACGCAGAAAGAGACAGT