Insertion junction: CLIP2.028226_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.028226
Chromosome:chromosome 2
Location:829184
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g800173 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCACAGGCCCCGGCACCGGCTATGAGCTGAAGATTTACAACCTCAAGGG
Internal bar code:AACGGTCCCTTATAGCGCTAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:510
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCAGTAGGTAGTGGAGGA
Suggested primer 2:ACAGGCGTGTCCACACTTAG