| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.028230 |
| Chromosome: | chromosome 2 |
| Location: | 6869702 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388615 | (1 of 1) K06965 - protein pelota (PELO, DOM34, pelA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCCAGTCAAGAACCCAAGTACCGATACCCAGTGCTGCCACCGCGCGTG |
| Internal bar code: | CCTGTCCCTGAAGGTAGTACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1717 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGATCATGAAACGACGCCA |
| Suggested primer 2: | TTGTGCTCATGCCATGCAAC |